All hardware, including ccd, heated cover assembly, peltiers, power supply and halogen. Relative quantitation of gene expression pn 4303859 send us your comments. It supports many realtime pcr applications for custom assays like gene expression, snp genotyping, viral load analysis, mirna qualification, and pathogen. Pdf use of applied biosystems 7900ht sequence detection.
The sds plate utility does not provide a gui to define reaction containers. The technician paired the 7900ht pcr with the pc workstation and sds software by starting a new experiment in the software. This is a secondgeneration sequence detection system designed for automated, highthroughput detection of fluorescent pcr related chemistries. Print bookmark share pdf 320kb english format file size language download get adobe reader. Technicians evaluation the technician paired the 7900ht pcr with the pc workstation and sds software by. Applied biosystems 7900ht fast realtime pcr system and sds enterprise database. Abi prism 7000 software download thermo fisher scientific. Abi applied biosystems 7900ht fast realtime pcr sequence. Quantitative realtime pcr protocol for analysis of nuclear. These files are designed to ensure easy and optimal data handling of qpcr data from the mircury lna universal rt microrna pcr system for users of instruments with sds collection software typically supplied with ab instruments.
Rt 2 profiler pcr arrays are compatible with all qiagen, abi, biorad, eppendorf, roche, and stratagene instruments. Applied biosystems 7900ht sequence detection system. Note, configure the new plate document, and enter the plate bar code by clicking tools document. Fast realtime pcr system and sds enterprice database system is used for fluorescentbased pcr chemistries to provide quantitative detection of nucleic acid sequences. It is designed for use with the abi prism 7900ht instrument and assaysondemandtm products. Health professionals are available to answer your questions, monday. The abi fast 7900ht realtime pcr instrument is an industry workhorse, in use for many years and still at the forefront in many laboratories around the world. Applied biosystems abi prism 7900ht sequence detection system system had an integrated thermal cycling block sold with original books, software disks, cables and computer. It is designed to support several pcr related chemistries. Abi prism, applied biosystems, celera, geneamp, primer express. Abi sds7900ht protocol realtime pcr core facility george hii, tel. The applied biosystems 7500 is todays top choice in rt pcr.
Your serial number is located on the back panel of your instrument. Afterwards, the sds software averages the spectrum recorded during the run and extracts the resulting spectral component to a calibration f ile. Applied biosystems 7900ht fast realtime system or abi prism 7900ht sequence detection system sequence detection systems sds software v2. Performing maintenance and thermo fisher scientific. Abi applied biosystems 7900ht fast realtime pcr system with. Applied biosystems 7900ht pcr sequence detection system w. Over 5,000 sample wells per day with 384well block configuration and standard thermal cycling protocol with automation accessory endpoint throughput. Rt 2 profiler pcr arrays are available in 96well plate, 384well plate. Applied biosystems 7900ht fast realtime pcr system 7900ht fast system. The applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide describes how to prepare, maintain, and troubleshoot the applied biosystems 7900ht fast realtime pcr system instrument, zymark twister microplate handler, and sds enterprise database. Abi applied biosystems 7900ht fast realtime pcr system. Personnel who maintain the 7900ht fast system hardware system administrators who maintain the 7900ht fast system software. Applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide. For customers th at do not generate platespecific container setup files with an auto mated software mechanism e.
If template is dna or cdna, plate is good for 3 days at room temperature protected from light and at least 1 week refrigerated. The applied biosystems 7900ht fast realtime pcr system is a sequence detection system instrument designed for automated, highthroughput detection of fluorescent pcr related chemistries. Qrdr mutation patterns for gonococcal samples as determined by direct sequencing and realtime pcr detection with the abi 7900ht sequence detection system advertisement content uploaded by justin. The automation accessory combined with 384well plate and 7900ht micro fluidic card capability make the 7900ht system ideally suited to meet the highthroughput requirements of todays drug discovery. Abi 7900ht7900htfast realtime pcr system a real asset to any disease research program, the abi 7900ht is the only realtime quantitative pcr system that combines 384well plate compatibility with. Applied biosystems 7900ht fast realtime pcr system user. Draft 512 applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide september 1, 2004 11. Assaysondemand gene expression products highcapacity cdna archive kit sybr green pcr master mix and rtpcr sds software tutorial.
Applied biosystems abi 7900ht pcr manufacturer specifications. The side access door was opened and closed, extending and retracting. Used applied biosystems 4330966 thermo abi 7900ht pcr. This abi prism 7900ht pcr unit features sequence detection in a 96well plate format.
Settings files for sds software settings files for sds software are available for download. The abi 7300 is great quantifying nucleic acid sequences without compromising data. Taqman fast universal pcr master mix 2, no amperase ung pn 4352042 getting started before you perform fast gene quantification, make sure that. The applied biosystems realtime pcr systems chemistry guide provides an easy. This is a secondgeneration sequence detection system designed for automated, highthroughput. Used applied biosystems thermo 7900ht pcr system lab. Dec 23, 2003 after the template titration assay run is complete, the abi 7900ht instrument software, sds2. Laser exposure the components of the abi prism 7900ht sequence detection system contain several lasers including an argon laser within the 7900ht instrument and a low power. Applied biosystems 7900ht sequence detection system grizzly. Applied biosystems abi 7500 real time pcr video youtube.
Applied biosystems 7900ht fast realtime pcr system software download. The applied biosystems 7900ht fast realtime pcr system is covered by u. Applied biosystems 7900ht pcr sequence detection system w pc. Text conventions this guide uses the following conventions. Applied biosystems 7900ht fast realtime pcr system and.
Audience this guide is intended for users of applied biosystems realtime pcr systems instruments and chemistries who have a wo rking knowledge of the polymerase chain reaction pcr process. Abi 7900ht 7900ht fast realtime pcr system a real asset to any disease research program, the abi 7900ht is the only realtime quantitative pcr system that combines 384well plate compatibility with fully automated robotic loading. Certified scientific instruments has a team of oem trained engineers who rebuild these instruments. The abi prism 7900ht sequence detection system is equipped with a blue laser and can be configured to have either 96 or 384 well blocks. Download your copy of the abi prism 7000 software please have your serial number available to enter on the registration form.
This small but robust pcr takes up less space than the applied. Fast realtime pcr system and sds enterprise database. The abi 7300 is a reliable tool which combines thermal cycling with fluorescence detection that measures cycle by cycle accrual. Abi sds 7900ht protocol realtime pcr core facility george hii, tel. Abi prism 7000 sequence detection systems relative. After the template titration assay run is complete, the abi 7900ht instrument software, sds2.
For running the plate using the template file, you need to open a template file. Also available is the abi 7900 rt pcr fully refurbished, calibrationalignment to factory standard. During the run, the 7900ht instrument performs a continuous scan of a background plate which contains pcr buffer or deionized water for 2 minutes at 60 c. Vantage services utilizing the abi 7900ht instrument. Manager of the sds software for use with the abi prism 7900ht sequence detection system. Manufacturer applied biosystems model 7900ht fast real. Keck center 7900 ht realtime quantitative pcr practical.
View online or download applied biosystems 7900ht user manual. Applied biosystems 7300 real time pcr system the abi 7300 is a reliable tool which combines thermal cycling with fluorescence detection that measures cycle by cycle accrual. The sds software analyzes the run data and displays the results in the results tab. Using the taqman rnase p instrument verification plate, the 7900ht system can distinguish between 5,000 and 10,000 template copies with 99. The 7900ht contains the hardware used for thermal cycling and detection of fluorescent chemistries. Applied biosystem 7300 real time pcr system andbio. Used abi 7900ht realtime pcr certified genetool, inc. Configuring the the software coordinates the action of the 7900ht instrument, the bar code reader, software and the plate handler while acquiring and saving raw data during each run. Applied biosystems 7900ht user manual pdf download. Applied biosystems 7900ht fast realtime pcr system absolute. The abi fast 7900ht realtime pcr instrument is an industry workhorse, in use for many years and still at the forefront in many laboratories around the. Applied biosystems 7900ht fast realtime pcr system and sds. The robot arm retrieves a plate and places it into the pcr. Find pricing on new and used applied biosystems 7900hts and other lab.
Applied biosystems 7900ht pcr sequence detection system. Applied biosystems 7900ht fast realtime pcr system software. Applied biosystems 7900ht fast realtime pcr system thermo. Used applied biosystems 4330966 thermo abi 7900ht pcr lab. Applied biosystems 7900ht fast realtime pcr system site preparation. Applied biosystems 7900ht fast realtime pcr system.
The sds plate utility software is designed for users who perform highthroughput analyses and need to generate sds plate documents automatically. Quantitative realtime pcr protocol for analysis of. The sds software sets gene expression levels for the calibrator sample to 1 log of zero. The sds software features two types of runs to support a variety of applications. Draft 512 applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide september. This guide also includes troubleshooting information for the maintenance procedures. Creating plate documents using the template batch utility. Oct 06, 2017 abi 7900ht thermo scientific 4329001 fast realtime pcr 4330966 w stacker 11291 duration.
Technicians evaluation the technician paired the 7900ht pcr with the pc workstation and sds software by starting a new experiment in the software. This small but robust pcr takes up less space than the applied biosystems 7900ht. Consequently, if more gene transcript is in the target sample kidney or. These files are designed to ensure easy and optimal data handling of qpcr data from the mircury lna universal. Feb 24, 2015 the abi 7500 is a real time pcr that delivers accurate results in less than 30 minutes. The default thermal cycler mode is set according to whether the computer is connected directly to the 7900ht instrument see below or the computer is offline see page 4. The applied biosystems 7900ht fast realtime pcr system is a sequence detection system instrument designed for automated, highthroughput detection of fluorescent pcrrelated chemistries it. Applied biosystems 7900ht fast realtime pcr system 4 user bulletin.
Abi prism 7900ht sequence detection system site preparation and safety guide pn 4317595 for an explanation of all the safety alert symbols provided in several languages. Data using realtime quantitative pcr and the 2ct method. The applied biosystems 7900ht fast realtime pcr system employs the automation controller software for automated operation of the 7900ht instrument. Applied biosystems 7300 real time pcr system andbio. Applied biosystems abi prism 7900ht realtime pcr system. The abi prism 7900ht sequence detection systemis a highthroughput realtime pcr system that detects and quantitates nucleic acid sequences. The video shows abi 7900ht pcr turned on with indicator lights illuminated. The abi 7500 is a real time pcr that delivers accurate results in less than 30 minutes. Applied biosystems 7900ht fast realtime pcr system relative.
Vantage offers realtime pcr services to assist users in verifying microarray results. Create a new plate document within the sds software by following the next steps. Flexible layout and controls rt 2 profiler pcr arrays are available in 96well plate, 384well plate, and 100well disc formats, and are used to monitor the expression of 84 or 370 genes related to a disease state or pathway. The automation accessory combined with 384well plate and 7900ht micro fluidic card capability make the 7900ht system ideally suited to meet the highthroughput requirements of todays drug discovery process. Our new yale medicineyale new haven health covid19 call center offers information on how to keep yourself and your family healthy. In order to begin a run, ensure the software is viewing an.